Cat. # | Size | Qty. | Price |
---|---|---|---|
30994S | 100 µl |
|
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 100 |
SOURCE | Rabbit |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human, Mouse, Rat
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CADM1 protein. Antibodies are purified by peptide affinity chromatography.
CADM1 (cell adhesion molecule 1, TSLC1, Necl-2, IgSF4, SynCAM1) is a member of the immunoglobulin (Ig) cell adhesion superfamily (1). It is a transmembrane protein that is primarily involved in mediating cell–cell adhesion (2,3). CADM1 is comprised of three extracellular Ig-like loops, a transmembrane domain, and a cytoplasmic tail that contains a 4.1 binding motif and a PDZ binding motif. The Ig-like loops contribute to cell-cell junction formation, whereas the cytoplasmic motifs provide structural linkage to the actin cytoskeleton and related adaptor proteins, including actin binding protein 4.1B and membrane-associated guanylate kinases (MAGUKs) (3-5). As an adhesion protein, CADM1 is involved in various biological processes in diverse cells and tissues (6). CADMI functions include structural maintenance and remodeling of epithelium, assembly of neuronal synapses, nerve-mast cell interaction, mast cell behavior, and T cell activation (3,7-9). In many solid tumors, CADM1 has been shown to function as a tumor suppressor by inhibiting malignant proliferation, invasion, and metastasis, while promoting tumor cell apoptosis. In some cases, however, CADM1 has been reported to promote tumor progression (6,10).
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