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9293
Bad Control Proteins

Bad Control Proteins #9293

Bad Control Protein (Non-phosphorylated): Nonphosphorylated Bad peptide fusion serves as a negative control. Supplied in SDS Sample Buffer.

Bad Control Protein (Phosphorylated): Phosphorylated Bad peptide fusion serves as a positive control. Supplied in SDS Sample Buffer.

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

  1. Yang, E. et al. (1995) Cell 80, 285-291.
  2. Zha, J. et al. (1996) Cell 87, 619-28.
  3. Datta, S.R. et al. (1997) Cell 91, 231-241.
  4. Peso, L. et al. (1997) Science 278, 687-689.
  5. Bonni, A. et al. (1999) Science 286, 1358-1362.
  6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.
  7. Harada, H. et al. (1999) Mol. Cell 3, 413-422.
  8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
  9. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
  10. Datta, S. et al. (2000) Mol. Cell 6, 41-51.
Entrez-Gene Id
572
Swiss-Prot Acc.
Q92934
For Research Use Only. Not For Use In Diagnostic Procedures.

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To Purchase # 9293S

Product Number Size
9293S 100 µl (10 western blots)

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Your Local Representative for Germany

New England Biolabs GmbH

Brüningstrasse 50, Geb. B 852

65926 Frankfurt am Main

Phone:
+49/ (0) 69 305 23140
Fax:
+49/ (0) 69 305 23149
Phone:
0800/246 5227 [Toll Free]
Fax:
0800/246 5229 [Toll Free]
Email:
info.de@neb.com
URL:
www.neb-online.de

Need information for a different country? Please click here.

To get local purchase information on this product, click here.