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For the best possible results, Cell Signaling Technology (CST) strongly recommends using our optimized application-specific protocols for each product. These protocols are the result of extensive in-house validation performed at CST and ensure accurate and reproducible results.

Product specific protocols will be linked from matching product web pages.

Western Blotting & Immunoprecipitation

Western Blotting Troubleshooting


Immunohistochemistry Troubleshooting


Flow Cytometry

Flow Cytometry Troubleshooting

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Troubleshooting

Sandwich Immunoassays (ELISA)

In-Cell Western