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We purchase our anti-IgM antibody from SouthernBiotech. It is Goat F(ab')2 Anti-Human IgM-UNLB (Cat. No.: 2022-01; https://www.southernbiotech.com/goat-f-ab-2-anti-human-igm-unlb-2022-01).
The Kinase Library can determine the kinases likely to phosphorylate any given motif. Learn about the research enabling this phosphoproteomics revolution.
Yes, our Protease/Phosphatase Inhibitor Cocktail (100X) #5872 can be used in mass spectrometry (MS) experiments. This cocktail does not contain AEBSF, which can cause peaks to shift.
PTMScan Motif Antibodies and Kits, for customers who purchase a license and would like to perform PTMScan Proteomics in-house.
The preparation of a cell lysate is crucial to the success of many assays. Proper storage and handling of the lysate are crucial to avoiding degradation and maintaining the ability to detect your protein.
This post explores common T cell types, their roles in modulating immune cell response, and T cell activation via interaction with cell surface receptors.
Information and case study data for the PTMScan Direct Cell Cycle and DNA Damage Service offered by CST.
The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3.
Jak and cytokine receptor mutations found in various cancers, along with the corresponding PubMed reference(s).
SignalStar™ Antibody Panel Builder allows you to easily select your custom panel's target species, antibodies, fluorophores, and imaging sequence.
The additional amino acids found in SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 should not affect the binding of RBD to ACE2 because the RBD region ends at amino acid 527.
What Assay should you be for Halloween? Western Blot, IHC, IF, Flow Cytometry, ChIP, or ELISA...
Cell Signaling Technology announces the launch of SignalStar™ Multiplex IHC, a flexible spatial biology solution for up to 8-plex amplification in two days.
A video tutorial illustrating the methodology behind PTMScan Technology and an example of its use in a recent phosphoproteomic study authored by scientists from CST.
The Jak/Stat Utilization Table displays the combinatorial use of tyrosine kinases and Stat proteins in cytokine/growth factor signaling.
Identifies and quantifies up to many hundreds of critical signaling nodes across pathways involved in cancer biology, immune cell signaling, and more.
A ChIP experiment uses 4M cells per reaction while a CUT&RUN assay only uses 5K to 100K starting cells, so it is expected to see a lower DNA yield from a CUT&RUN assay than from ChIP experiments. We have found that digestion at 4C can increase not only the signal strength but also the DNA yield. DNA purification with phenol/chloroform can also increase the DNA yield but it also can increase the background, so we do not recommend it. Always start with more cells if you can, and make sure that your cells are healthy. Please note that a high specificity of the DNA sample and not a high DNA yield is what you need to pursue for a good result.
Download white paper guides to key CST applications: western blotting and iPSC generation.
A binary approach is one of the best ways to evaluate antibody specificity.
CUT&Tag Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
Overview of methodology and client deliverables for the PTMScan Direct Proteomic Service offered by Cell Signaling Technology.
Yes, you can use BSA #9998 as a carrier for cytokines. It should be dissolved into 1X PBS and sterile filtered.
Mass spectrometry is a highly sensitive technology used to study proteins and their PTMs in a sample on a large scale.
Our Akt Antibody #9272 detects all 3 isoforms of Akt: Ak1, Akt2, and Akt3.
Validation using recombinant proteins or exogenous expression in a surrogate cell line can be used for targets with low levels of protein expression.
Here’s what you should know about your target before you start searching for antibodies, and the questions to ask to avoid choosing the wrong one.
Although publications usually recommend pair-end sequencing for CUT&RUN assay, we find that single-end sequencing works as well. Which one to choose depends on if you would like to know the size of the CUT&RUN DNA fragments, as this information is only provided by pair-end sequencing. If you are only interested in the genomic binding loci of the target protein, but not the length of each DNA fragment by MNase digestion, then single-end sequencing is fine and cost-effective.
CST is proud to partner with global green chemistry nonprofit Beyond Benign to support the creation of green chemistry teaching resources for STEM education.